Journal: Acta Materia Medica

Article Title: Cryptotanshinone alleviates vasculitis in Kawasaki disease by modulating macrophage-neutrophil interactions

doi: 10.15212/amm-2024-0076

Figure Lengend Snippet: Figure 1 | CTS alleviates disease severity in the LCWE mouse KD model. A. Schematic diagram of KD model construction in LCWE-induced KD mice. B. Body temperatures of mice in the normal, LCWE, high-dose CTS, and low-dose CTS groups during 2 weeks of model construction. C. Representative images of the abdominal area in the four groups of mice, and statistical analysis of abdominal aortic diameter. Scale bars, 200 mm. D. Representative H&E images of heart tissue, coronary arteries, and aortic root regions in the four groups of mice. Scale bars, 50 μm.

Article Snippet: For immunohistochemistry, mouse heart tissue paraffin sections were deparaffinized, rehydrated, subjected to antibody retrieval with sodium citrate, blocked, stained with anti-F4/80 antibody (Cell Signaling Technology, cat. no. 30325T) at a dilution of 1:100, and incubated overnight at 4°C.

Techniques:

Journal: Acta Materia Medica

Article Title: Cryptotanshinone alleviates vasculitis in Kawasaki disease by modulating macrophage-neutrophil interactions

doi: 10.15212/amm-2024-0076

Figure Lengend Snippet: Figure 5 | CTS treatment decreases macrophage-neutrophil interactions in mouse heart tissue.

Article Snippet: For immunohistochemistry, mouse heart tissue paraffin sections were deparaffinized, rehydrated, subjected to antibody retrieval with sodium citrate, blocked, stained with anti-F4/80 antibody (Cell Signaling Technology, cat. no. 30325T) at a dilution of 1:100, and incubated overnight at 4°C.

Techniques:

Journal: Biology of reproduction

Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.

doi: 10.1095/biolreprod.106.059410

Figure Lengend Snippet: FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

Article Snippet: Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.

Techniques: Western Blot, Control, Positive Control, Transformation Assay